New ara-C resistant mutants of the Chinese hamster ovary cells
نویسندگان
چکیده
منابع مشابه
Podophyllotoxin-resistant Mutants of Chinese Hamster Ovary Cells
Mutants of Chinese hamster ovary cells which have been obtained after one or two selection steps in the presence of the microtubule inhibitor podophyllotoxin (PodR1 and Podm' mutants, respectively) and which exhibit very specific types of cross resistance (and/or collateral sensitivity) towards various other inhibitors of microtubule assembly (e.g. colchicine, colcemid, stegnacine, vinblastine,...
متن کاملPodophyllotoxin-resistant mutants of Chinese hamster ovary cells. Alteration in a microtubule-associated protein.
Mutants of Chinese hamster ovary cells which have been obtained after one or two selection steps in the presence of the microtubule inhibitor podophyllotoxin (PodR1 and Podm' mutants, respectively) and which exhibit very specific types of cross resistance (and/or collateral sensitivity) towards various other inhibitors of microtubule assembly (e.g. colchicine, colcemid, stegnacine, vinblastine,...
متن کاملHyperthermic enhancement of cell killing by mitomycin C in mitomycin C-resistant Chinese hamster ovary cells.
The interaction of hyperthermia and mitomycin C (MMC) was studied in wild-type Chinese hamster ovary cells and in Chinese hamster ovary cells selected for resistance to MMC. Hyperthermic potentiation of MMC activity was maximal if heating was done simultaneously, or up to 3 h following MMC exposure. Heat enhanced the effect of MMC in both drug-sensitive and -resistant cells. Dose enhancement ra...
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Proteinase 3(PR3) is a human polymorphonuclear leukocyte serine proteinase and is the main target antigen for antineutrophil cytoplasmic antibodies (ANCA) found in Wegener's granulomatosis (WG). We developed a stable expression system for conformationally intact recombinant PR3 (rPR3) in Chinese hamster ovary cells (CHO-cells). The part of PR3 cDNA that encoded the active form of PR3 was s...
متن کاملIntracellular Localization of FLAG-Peroxisomal Protein in Chinese Hamster Ovary (CHO) Cells
Epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant DNA techniques. The aim of this study was to sub-clone the peroxisomal protein (PEP) cDNA into a mammalian expression vector leading to the formation of a chimeric PEP-cDNA containing the FLAG epitope. The FLAG-PEP recombinant cDNA was construc...
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ژورنال
عنوان ژورنال: Genetical Research
سال: 1985
ISSN: 0016-6723,1469-5073
DOI: 10.1017/s0016672300022254